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Post-doctoral position on developmental genetics

A post-doctoral position is proposed at the Institute of Research in Agriculture and Seeds (IRHS, Angers) to work on rose development. The objective of the project is to study the role and the regulation of TFL1/FT homologues during blooming and bud-outgrowth.

Rosier Old Blush © N.Mansion, Inra
Updated on 06/12/2014
Published on 04/18/2014

A post-doctoral position is proposed at the Institute of Research in Agriculture and Seeds (IRHS, Angers) to work on rose development. The objective of the project is to study the role and the regulation of TFL1/FT homologues during blooming and bud-outgrowth.

Involvement of TFL1 (TERMINAL FLOWER 1)/FT (FLOWERING LOCUS T) homologue genes in rose development.

Location: Angers, France
Expected starting date:  Sept 2014
Duration:  18 months

Research proposal description    
Rose is the most important economical ornamental plants. Controlling rose development and blooming is an important issue for rose productions in garden roses or cutting flower sectors. The GDO team has demonstrated the importance of TERMINAL FLOWER 1 / FLOWERING LOCUS T homologues in the control of blooming in rose. RoKSN, a TFL1 homologue, is a major regulator of continuous flowering in rose and is regulated by gibberellins, GA. The ArchE team has studied in detail the physiological and molecular bases of bud outgrowth in rose in response to exogenous (light, temperature) and endogenous (hormones, sugars) stimuli. Recent findings support that the FT/TFL1 gene family, a small family of 6 genes, is also involved in other aspects of plant development.
In this project, we proposed to combine the knowledge of both teams on blooming and bud-outgrowth to study the regulation of FT/TFL1 homologues in rose during these processes and their controls by exogenous and endogenous factors. As these genes are known to interact with transcription factors, we want to identify by screening of a two hybrid bud rose library, the protein partner with which they interact to better understand the mode of action.

Expertise and competences
Functional genetics, molecular physiology, plant development (especially in Arabidopsis thaliana).

Hosting conditions and research facilities
The candidate will be hosted at IRHS where he/she will have access to all facilities for molecular and physiological studies and plant growth (access to greenhouse). Both teams have expertise in functional genetics, protein interaction, gene expression and physiology, techniques and competences necessary for the success of this project. He / she will use the different techniques developed by the teams such as in situ hybridisation, RT-qPCR, yeast-two-hybrid, transgenesis. The ArchE team has developed an in vitro system to study bud response to different factors and a two-hybrid rose bud librairy.
Concerning plant materials, the candidate will use the plant material produced by both teams and already available: tissues during bud outgrowth and blooming process, tissues obtained in different environmental conditions (light, dark, temperature) or after exogenous application of hormones (gibberellins, sugar, auxin).
IRHS is member of the SFR QUASAV. The candidate will have access to the different platforms of the SFR as the IMAC (for histological studies) or ANAN (for nucleic acid studies) platform.

Contact
Fabrice Foucher : fabrice.foucher@angers.inra.fr
Soulaiman Sakr : soulaiman.sakr@agrocampus-ouest.fr